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BV RAPID BLUE™Bacterial Vaginosis Detection Kit (Sialidase)

Detection Principle

Bacterial Vaginosis (BV) is caused by the disorder of normal vaginal flora. Anaerobic bacteria are pathogenic bacteria, mainly including:

> Gardnerella vaginalis

> Prevotella spp

> Bacteroides spp

All of these anaerobic bacteria can secrete sialidase, and the overall level of sialidase secreted by these anaerobic bacteria exceeds a certain threshold during the induction of BV.

This diagnostic reagent is designed based on the principle that vaginal secretions of female individuals with BV would present abnormal sialidase activity. Clear and simple indications are provided via this detection method. A blue color (including green) indicates a positive result, while a yellow color indicates a negative result.

Detection Method and Diagram

1. Collect the secretion samples from the vaginal wall (middle section) of a patient with a cotton swab (as many as possible). Take out a test tube and unscrew its cap.

2. Dip the sampling cotton swab into the test tube solution, and stir and shake it properly.

3. Put the test tube with the cotton swab in a thermostat at 37℃ for 10 min.

4. Take out the chromogenic reagent bottle and unscrew its cap. Then, add one or two dropschromogenicreagent to the test tube containing a cotton swab, and shake it properly.

5. Observe the color immediately within 3 min after the chromogenic reagent is added.

Result Interpretation (diagram):

a) Positive result: A blue or green color in the test tube or on the cotton swab indicates a positive result.

B) Negative results: A yellow color in the test tube indicates a negative result.

Storage and Stability

The BV RAPID BLUE™ detection kit shall be stored at 4℃-26℃ and used before the expiration date.

Result Reliability

Sensitivity: 92.7% Specificity: 96.9% Total Accuracy: 95.8%

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